Name: Barbara C.

e-mail: mw1.barbara@gmail.com

Work experience

May 2009 – onwards: part-time job as Research technician at University of Padua (Italy)

My activity involves:

• Full custom gene expression profiling using microarray technology and qRT-PCR
• Bioinformatic and statistical analysis of microarray and qRT-PCR data
• Scientific and technical support to academic/non academic laboratories
• Customer relationship management

January 2009 – April 2010: Research Fellow at ULSS 18 – Rovigo

Research interests and activities: identification of diagnostic and prognostic marker genes – lung cancer – qRT-PCR - statistical analysis.

January 2000 – December 2008: Research Fellow at University of Padua

Research interests and activities: identification of diagnostic and prognostic marker genes – functional genomics - neuromuscular deseases – genetics and physiology of skeletal muscle - in vitro cellular models – model organisms - gene expression profiling – technological improvement - microarray technology – qRT-PCR – bioinformatic and statistical analysis - writing scientific papers – power point presentations.

July 1998 – October 1999: Undergraduate student at University of Padua

Lab Training in molecular biology, microsatellite analysis and sequencing.

Research interests: population genetics applied to environmental issues and conservation of endemic species.

Education

August 2003-July 2005: Post-doctoral position at University of Padua (Italy)

March 2003: Ph.D in Genetics at University of Ferrara (Italy), funded by Telethon grant B41

November 1999: Bachelor’s Degree in Biological Sciences (specialization in environmental biology) at University of Padua (Italy) (110/110)

July 1992: High school degree, Liceo Scientifico Statale (Italy) (58/60)

Short term training courses

2005: English training course for writing scientific papers, 15 hours.

Organized by Dept. of Biology, University of Padua

Teaching activiy

February 2006: Introduction to molecular biology techniques (4 days)

Organized by Dept. of Biology, University of Padua

Molecular and Cellular Biology skills

Good skills in several molecular and genetic techniques: total RNA, DNA and protein isolation, nucleic acids purification and quality control; quantitative RT-PCR; cDNA and oligonucleotide microarray technology; qualitative PCR and agarose gel electrophoresis.

Familiarity with: in vitro human primary myoblasts culture, differentiation and characterization; myoblasts purity assessment and cell sorting; immunofluorescence, immunocytochemistry.

Basic knowledge of acrilamide gel electrophoresis and analysis of microsatellite markers using semi-automatic sequencer.

Informatics, bio-informatics and statistics

Good knowledge of Office (2000-XP), Internet/www, Mozilla Firefox, CorelDraw ver.10.0 and specific softwares for image analysis; basic knowledge of HTML4.

Nucleotide sequence similarity searches with BLAST and BLAT. Analysis of promoter regions and identification of putative transcription factors binding sites.

Statistical analysis of data obtained from microarray and qRT-PCR experiments using dedicated packages.

Mother tongue

Italian

Other Languages

English, intermediate level. Excellent written and good oral understanding of scientific English.

Personal skills

Able to present technical data, understands grant and proposal writing, able to concisely summarize large bodies of information, work on a team, confident in handling new tasks, flexible in assignments.

Publications

1. Romualdi C., Giuliani A., Millino C., Celegato B., et al. (2009).Correlation between gene expression and clinical data through linear and nonlinear principal components analyses: muscular dystrophies as case studies.OMICS. 2009 (3):173-84.

2. Celegato B., Capitanio D. et al. (2006). Parallel protein and transcript profiles of FSHD patient muscles correlate to the D4Z4 arrangement and reveal a common impairment of slow to fast fibre differentiation and a general deregulation of MyoD-dependent genes. Proteomics 6(19) 5303-21.

3. Romualdi C., Trevisan S., Celegato B. et al. (2003). Improved detection of differentially expressed genes in microarray experiments through multiple scanning and image integration. Nucleic Acids Res. 31(23):e149.

4. Campanaro S., De Pittà C., Celegato B. et al. (2003) Application of a cDNA microarray for the analysis of muscular dystrophies and childhood leukemias. Minerva Biotec. 15, 235-244.

5. Campanaro S., Romualdi C., Fanin M., Celegato B. et al. (2002). Gene expression profiling in dysferlinopathies using a dedicated muscle microarray. Hum. Mol. Gen. 11(26) 3283-98.

6. Campanaro S., Romualdi C., De Pittà C., Fanin M., Celegato B. et al. (2002). Gene Expression Profiling in Skeletal Muscle Using a Dedicated Microarray. Minerva Biotechnologica, 14, 273-279.

7. Romualdi C., Campanaro S., Campagna D., Celegato B. et al. (2003). Pattern recognition in gene expression profiling using DNA array: a comparative study of different statistical methods applied to cancer classification. Hum. Mol. Gen. 12(8) 823-836.