Name: Barbara C.
May 2009 – onwards: part-time job as Research technician at University of Padua (Italy)
My activity involves:
January 2009 – April 2010: Research Fellow at ULSS 18 – Rovigo
and activities: identification of diagnostic and prognostic marker genes
– lung cancer – qRT-PCR - statistical analysis.
January 2000 – December 2008: Research Fellow at University of Padua
Research interests and activities: identification of diagnostic and prognostic marker genes – functional genomics - neuromuscular deseases – genetics and physiology of skeletal muscle - in vitro cellular models – model organisms - gene expression profiling – technological improvement - microarray technology – qRT-PCR – bioinformatic and statistical analysis - writing scientific papers – power point presentations.
Lab Training in molecular biology, microsatellite analysis and sequencing.
Research interests: population genetics applied to environmental issues and conservation of endemic species.
2005: Post-doctoral position at University of Padua (Italy)
Ph.D in Genetics at University of Ferrara (Italy), funded by Telethon
Bachelor’s Degree in Biological Sciences (specialization in environmental
biology) at University of Padua (Italy) (110/110)
July 1992: High school degree, Liceo Scientifico Statale (Italy) (58/60)
2005: English training course for writing scientific papers, 15 hours.
Organized by Dept. of Biology, University of Padua
February 2006: Introduction to molecular biology techniques (4 days)
Organized by Dept. of Biology,
University of Padua
Good skills in several molecular and genetic techniques: total RNA, DNA and protein isolation, nucleic acids purification and quality control; quantitative RT-PCR; cDNA and oligonucleotide microarray technology; qualitative PCR and agarose gel electrophoresis.
Familiarity with: in vitro human primary myoblasts culture, differentiation and characterization; myoblasts purity assessment and cell sorting; immunofluorescence, immunocytochemistry.
Basic knowledge of acrilamide gel electrophoresis and analysis of microsatellite markers using semi-automatic sequencer.
Good knowledge of Office (2000-XP), Internet/www, Mozilla Firefox, CorelDraw ver.10.0 and specific softwares for image analysis; basic knowledge of HTML4.
Nucleotide sequence similarity searches with BLAST and BLAT. Analysis of promoter regions and identification of putative transcription factors binding sites.
Statistical analysis of data obtained from microarray and qRT-PCR experiments using dedicated packages.
English, intermediate level. Excellent written and good oral understanding of scientific English.
Able to present technical data, understands grant and proposal writing, able to concisely summarize large bodies of information, work on a team, confident in handling new tasks, flexible in assignments.
1. Romualdi C.,
Giuliani A., Millino C., Celegato B., et al. (2009).Correlation between
gene expression and clinical data through linear and nonlinear principal
components analyses: muscular dystrophies as case studies.OMICS. 2009
2. Celegato B.,
Capitanio D. et al. (2006). Parallel protein and transcript profiles
of FSHD patient muscles correlate to the D4Z4 arrangement and reveal
a common impairment of slow to fast fibre differentiation and a general
deregulation of MyoD-dependent genes. Proteomics 6(19) 5303-21.
3. Romualdi C.,
Trevisan S., Celegato B. et al. (2003). Improved detection of differentially
expressed genes in microarray experiments through multiple scanning
and image integration. Nucleic Acids Res. 31(23):e149.
4. Campanaro S.,
De Pittà C., Celegato B. et al. (2003) Application of a cDNA microarray
for the analysis of muscular dystrophies and childhood leukemias. Minerva
Biotec. 15, 235-244.
5. Campanaro S.,
Romualdi C., Fanin M., Celegato B. et al. (2002). Gene expression profiling
in dysferlinopathies using a dedicated muscle microarray. Hum. Mol.
Gen. 11(26) 3283-98.
6. Campanaro S.,
Romualdi C., De Pittà C., Fanin M., Celegato B. et al. (2002).
Gene Expression Profiling in Skeletal Muscle Using a Dedicated Microarray.
Minerva Biotechnologica, 14, 273-279.
7. Romualdi C., Campanaro S., Campagna D., Celegato B. et al. (2003). Pattern recognition in gene expression profiling using DNA array: a comparative study of different statistical methods applied to cancer classification. Hum. Mol. Gen. 12(8) 823-836.